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KMID : 0380520030070010001
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Korean Journal of Veterinary Pathology
2003 Volume.7 No. 1 p.1 ~ p.4
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Development of dot blot hybridization method using non-radio labeled probes for the diagnosis of malignant catarrhal fever
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Kim Ok-Jin
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Abstract
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Malignant catarrhal fever (MCF) is a systemic disease of ruminants caused by a gamma herpesvirus, ovine herpesvirus 2 (OvHV-2). Dot blot hybridization (DBH) protocols for detecting and differentiating this MCF virus were developed. OvHV-2 specific primer pairs, 556/555, were used for the amplification of target DNA. Then, the amplified DNA was labeled with incorporation of digoxigenin (DIG). The Dig-labeled probe was able to detect and differentiate specifically OvHV-2 DNA. This DBH technique can be applied to confirm the presence of MCF virus on clinical samples and to differentiate specifically between OvHV-2 infection and other viral infections.
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KEYWORD
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dot blot hybridization, malignant catarrhal fever, ovine herpesvirus 2
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